Microplate Reader. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. #N207) LAL Reagent Water (Lonza cat. 2 with 1000 cells. 17,000 cells. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. $488. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Click SoftMax Pro Software User Guide. What are the dimensions of the plate? Code. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. one 96-well sample plate can be controlled from 15–40°C,. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Plate chamber design of the SpectraMax category 1 microplate readers. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The picked. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. 7°C (gradient heating design). 96-well microplate compatible Well read area range: D4. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. 0% ± 0. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. It features a modular design that allows users to add or change detection cartridges according to their needs. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Thermomax model provides thermal control and vertical shake, perfect for. • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Important things to consider when purchasing a multimode microplate reader. 384-WELL SBS STERILE MICROPLATE. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. µl) was added to each well and incubated at 37°C for 1 h. More Product Information. • 0. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. • Easy and gentle immobilization of biotin. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Compare this item. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. S. These plates are compatible with standard fluorescence microplate readers and are offered in a. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. An example of dual-luciferase assay results. In addition, the iD5 reader measures TRF and FP and. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Published Dec 16, 2020 | Updated Dec 16, 2020. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Packed 25 plates per…. 100 μL of working reagent was added to each well after the 10-minute preincubation step. 1% in white 96- and < 0. 5 OD at 492 nm Computer interface USB 2. Four wash/rinse bottles are included and configurable for both 96- and 384. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. Categories: Plate Readers. x. 5 second read per plate. 3 OD at 200 nm. Published Dec 16, 2020 | Updated Dec 16,. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. and the A1 corner of the plate adapter (Figure 1-2). UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. 0 . Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. 1. Washer maintenance kit: 2 X T 2. This reader also makes use of a unique temperature-independent methodology for path length correction. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. It offers extremely low absorbance background, excellent. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. with Quartz ABS Plastic. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Validation made easy -1) Select the correct plate and the wells that you want to measure. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). TNF standard curve. Shaking: Linear, orbital, and double orbital. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The CellTiter-Glo assay was used to measure ATP. Three low volume 24-well bottom slides. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Instrument settings can. REF (SpectraMax. Ensure that cells reach around 80–90% of confluence on the day of the. 2. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). No accessories are needed and SoftMax® Pro Software. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. B. Description. Turn on Plate Reader and wait for it to complete its start-up routine. SpectraMax i3x Multi-Mode Microplate Reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Microplate readers, SpectraMax®, with SpectraDrop. Page 1 of 8 . The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Growth medium was then replaced with fatty acid uptake solution. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Microplate Spectrophotometers. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. Reconstitution of. ©2022 Molecular Devices - All rights. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Sample by eye within . S. 25% and 0. 190. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Or scan up to 384 wells at any time to determine the best assay conditions. Two high-energy xenon flash lamps: Reading capabilities. Well scanning With well scanning, multiple points within each well can be read, providing a high. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. NanoQuant plate. #3693; n = 4 for each standard). 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. adjust the reader. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. Inconsistent results: cannot determine whole well confluence – well after well. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. FlexIII. Molecular Devices SpectraMax Plus 384 Absorbance. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. A = Log 10 (I 0 /I). Gemini XPS Reader scans. The variations include adjustable. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. Fluorescence Microplate Reader Comparison Chart. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 2% in. Microplate Readers. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. Expand Microplates and select the newly created plate. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. x. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Fluorescence Microplate Reader Comparison Chart. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Please click on the links below to view the Microplate Reader Comparison Tables. Microplate format. Service Contracts, Preventative Maintenance and. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Dual injectors for kinetic assays. HeLa. as well as all other Molecular Devices' microplate readers. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. Microplate Capacity. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. Click Create. Temperature variation ± 0. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. x. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 1. 000 – 4. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Microplate Readers include the M3, M4, M5, and M5e models. x. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Published Dec 16, 2020 | Updated Dec 16, 2020. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. absorbance microplate reader GEA. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. 5% at 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. x. EMax. Plus 384. Absorbance cuvette port. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). On the day of the assay, the plate was equilibrated. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. 96- or 384-well microplate compatibility. maximum-illumination-channels. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. 6. Polypropylene deep well plates, round bottom. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Mix samples in microplate wells by user-programmable Automix feature. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. style. Next generation SpectraMax® absorbance microplate readers. Molecular Devices' microplate readers. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. D. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. 00. White 96-well platePlate formats. (Molecular Devices cat. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Products : General, FilterMax F5 reader. Top or bottom detection is available with a simple click of a button. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Working in. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 5 ng per well. These robust readers have been placed in labs from. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. perfect for kinetic readings involving growth curves at 5 seconds per plate. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Capabilities. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Optional 16-way manifold. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. 3. 304 signal level saturation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Loading Buffer was also added to a set of cell-free wells for background controls. Populate the following info under Description. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Consequently, FLIPR has been used. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. White 96-well plate Plate formats. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. MCF7 cells treated with. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Top and bottom reads are. Highly selective for dsDNA over ssDNA or RNA. These microplates have a corner notch for use in standard plate readers. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. Uncontrolled Copy for Training and Reference Purposes Only . x. to each well. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. 012 fmol 0. #ABS PLUS) Methods. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Contents of wells were pipetted up and down several times to mix. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. 303 unable to cal dark current. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Supplier: Molecular Devices. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. SoftMax Pro GxP Software version 5. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Top and bottom reads are. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. This can increase throughput and efficiency, thus saving time and space in the lab. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Multimodal plate reader: Yes: 25 mm: Yes—Max. The plate reader optics are tunable for. validation, maintenance, and repair documentation. Selects either 96-well or 384-well plate for a stand-alone read. Some will only support one well density (e. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. #ABS Plus) Cell plating and treatment prior to assay. . a white 96-well half-area microplate (Corning cat. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. 0025 OD from 0. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Select up to 3 products. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. High tolerance for contaminants. 340 PC384. Dimensions: 18. 0 OD. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Artel MVS Bundle DM Std/800TSNB Reader Details. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Microplate optimization and read height optimization were performed for best results. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Absorbance Linearity: 2. Browse for different fluorescent readers below and compare across different instrument suppliers. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The color touch screen provides a visual user interface, making programming fast and intuitive. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Plate and Setup Rack Options Screens with selection of up to four Methods shown. They can be read by most microplate readers. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. Plate Shaking. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Published Dec 16, 2020 | Updated Dec 16, 2020. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. SpectraMax® Microplate Reader Suite of Products Knowledge Base. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. UV absorbance detection. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. x. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Microplates, cuvettes (via adapter) Detectors. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). 2. Compact designs make these instruments. variety of. No accessories are needed and SoftMax® Pro Software. This reader can also be used as a standard. Infinite possibilities. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Unparalleled performance on a personalized platform. 5 fg per well to 0. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 2. (one set per assay) to clear 96-well microplates. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. 000 to 2. FAST AND ACCURATE METERTECH Microplate Reader $3,650. 6-384 (96- and 384-well with pipetting) 96-well microplates. The opaque walls prevent stray light from being detected from neighbouring. A standard 96 well plate was set up as described in Protocol Table 4. Cross-talk <0. * Note: Programming the washer with a final aspirate height of 4.